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Pyruvate kinase
Definition
Pyruvate kinase is an enzyme found in red blood cells that helps change glucose to energy when oxygen is low.
This article discusses the test to measure the level of pyruvate kinase in your blood.
Why the Pyruvate kinase is Performed
This test is done to detect abnormally low levels of pyruvate kinase. If you do not have enough of this substance, red blood cells break down faster than normal. This can lead to hemolytic anemia.
See also: Pyruvate kinase deficiency (PKD)
How the Pyruvate kinase is Performed
Blood is drawn from a vein, usually from the inside of the elbow or the back of the hand. The site is cleaned with germ-killing medicine (antiseptic). The health care provider wraps an elastic band around the upper arm to apply pressure to the area and make the vein swell with blood.
Next, the health care provider gently inserts a needle into the vein. The blood collects into an airtight vial or tube attached to the needle. The elastic band is removed from your arm.
Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding.
In infants or young children, a sharp tool called a lancet may be used to puncture the skin and make it bleed. The blood collects into a small glass tube called a pipette, or onto a slide or test strip. A bandage may be placed over the area if there is any bleeding
In the laboratory, white blood cells are removed because they can alter test results, and the level of pyruvate kinase is measured.
How to Prepare for the Pyruvate kinase
If your child is to have this test performed, it may be helpful to explain how the test will feel, and even demonstrate on a doll. Explain the reason for the test. Knowing the "how and why" may reduce the anxiety your child feels.
How the Pyruvate kinase Will Feel
When the needle is inserted to draw blood, you may feel moderate pain, or only a prick or stinging sensation. Afterward, there may be some throbbing.
Risks
Veins and arteries vary in size from one patient to another and from one side of the body to the other. Obtaining a blood sample from some people may be more difficult than from others.
Other risks associated with having blood drawn are slight but may include:
- Excessive bleeding
- Fainting or feeling light-headed
- Hematoma (blood accumulating under the skin)
- Infection (a slight risk any time the skin is broken)
Normal Results
Results vary depending upon the testing method used.
What Abnormal Results Mean
Low levels confirm pyruvate kinase deficiency.
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Typical mistypes for Pyruvate kinase
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